Table 3 Cross-linked sites observed with different protein:BS3 (d0/d4) ratios for the complex Bcd1pFL:Rtt106p-M.

From: The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity

Cross-linked proteins

Linkage sites

Ratio 1:50

Ratio 1:100

Distancea Cα–Cα (Å)

Bcd1pFL–Bcd1pFL

K16–K62

 

√

N.C.

K16–K88

√

√

N.C.

K16–S106

√

 

N.C.

K16–K109

  

N.C.

K16–K151

 

√

N.C.

K16–K258

√

√

N.C.

K62–K80

 

√

N.C.

K80–K151

√

 

N.C.

K87–K151

√

 

N.C.

K140–K151

√

 

15.4

K188–K151

√

√

10.1

Rtt106p-M–Bcd1pFL

S177–K151

√

√

19.4

  1. Identified cross-linked sites are represented for all tested ratios (technical duplicates).
  2. aCα–Cα distances are indicated according to the crystal structure (N.C. for “not crystallized” mention is indicated for cross-links involving at least one site that has not been crystallized).
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