Fig. 5: Cytoplasmic dsRNA causes robust, sustained p-IRF3(S396) and ISG expression. | Nature Communications

Fig. 5: Cytoplasmic dsRNA causes robust, sustained p-IRF3(S396) and ISG expression.

From: Viral infection of cells within the tumor microenvironment mediates antitumor immunotherapy via selective TBK1-IRF3 signaling

Fig. 5

a, b Immunoblot analysis of innate signaling in MDMs after treatment with PVSRIPO (MOI 10), LPS (100 ng/ml), or IFNα2 (200 IU/ml) over time. Additional analyses from four donors, including those quantitated in (b), are presented in Supplementary Fig. 8. b %Max densitometry values for each protein/phospho-protein followed by subtraction of zero time point is shown. (†) Significant paired t test (two-tailed) comparing maximum value (across time course) induced by PVSRIPO (blue) vs LPS (red); IRF3-p (S396) p = 0.0001, IFIT1 p = 0.01, OAS1 p = 0.009, p50 p = 0.008, p100 p = 0.001, p105 p = 0.01, A20 p = 0.006. c Comparison of PVSRIPO, Poly(I:C), Poly(I:C)-tfx, and LPS treatment using respective low and middle doses presented in Fig. 4c by immunoblot for relevant proteins. Three donors were tested, p-IRF3(S396) blots are shown for all donors. d DCs were treated with mock, PVSRIPO, Poly(I:C) or LPS along a time course for immunoblot analysis as shown; n = 3 experiments.

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