Fig. 1: CSDE1 is a positive modulator of VSV replication.

A Hep3B cells were transfected with no siRNA, Negative control siRNA, or with [s15373 + 15374 siRNA] (2 CSDE1-specific siRNA)⁴⁶ and levels of CSDE1 assayed by western blotting 24 or 48 h later. (Representative of three separate experiments). B–D Forty-eight hours following transfection with siRNA as in A, Hep3B cells were infected with VSV-GFP (MOI 0.1). Forty-eight hours (B) or 96 h (C) later, viral titers were determined by plaque assay and D the number of surviving cells was counted at 96 h post infection. Representative of two separate experiments. E B16-, B16-CSDE1^C-T-, or B16-CSDE1^WT-overexpressing cells were infected with VSV-IFN-β at an MOI of 0.1. Twenty-four, 48, and 72 h later, viral titers were measured on BHK cells by plaque assay. Representative of three separate experiments. F Parental Hep3B cells or pooled populations of Hep3B-overexpressing wild-type CSDE1^WT, or mutant CSDE1^C-T, were infected with VSV-IFN-β (MOI 0.1) (3 wells/group). Forty-eight hours later (Passage 1), supernatants were assayed for infectious titers on the same cells on which the virus was passaged. Virus was recovered every 48 h (P2–5) and similarly titered. Representative of three separate experiments. G Stock VSV-IFN-β virus or VSV-IFN-β, which had been passaged five times through Hep3B parental or Hep3B-CSDE1^C-T cells as in F, was titered on either Hep3B parental cells or on Hep3B-CSDE1^C-T cells. Representative of two separate experiments. Means ± SD of three technical replicates are shown. P-values were determined using a one-way (B–D) or two-way (E–G) ANOVA with a Tukey’s multiple comparisons post test on log-transformed data. Statistical significance was set at p < 0.05, ns > 0.05. Source data are provided as a Source Data file.