Fig. 2: Forced evolution of VSV selects a single point mutation in the P/M IGR. | Nature Communications

Fig. 2: Forced evolution of VSV selects a single point mutation in the P/M IGR.

From: Oncolytic virotherapy induced CSDE1 neo-antigenesis restricts VSV replication but can be targeted by immunotherapy

Fig. 2

VSV-IFNβ was passaged 5× through Hep3B or Hep3B-CSDE1C-T cells as in Fig. 1. Sanger sequence of the IGR between the P and M genes (A) from virus populations passaged through B Hep3B parental cells showed homogenous populations of wild-type sequence; sequence of viruses passaged through either C Hep3B-CSDE1C-T cells or D Hep3B-VSV-IFNβ−21d ESC cells were largely homogenous for a point mutation C–U. Representative of five separate experiments. E Virus population from a Hep3B tumor grown in a SCID mouse and excised upon recurrence following treatment with VSV-IFNβ was a heterogenous population of wild-type and mutant IGR P/M viruses. Representative of two separate escape tumors.

Back to article page