Fig. 4: Allele-specific genetic dependencies in COAD cell lines.
From: The origins and genetic interactions of KRAS mutations are allele- and tissue-specific
a Gene sets with significant enrichment for increased (lower dependency score; purple) or reduced (higher dependency score; orange) genetic dependency in COAD cell lines. The size of the dot relates the FDR-adjusted p value of the association and the color indicates the strength of the enrichment (“normalized enrichment score”). b, c Heatmaps ranking the cell lines by dependency (“dep.”) score of the genes at the leading edge of enrichment for two gene sets. Each row represents a gene and each cell represents a cell line colored by its KRAS allele. The cell lines are arranged in ranking order by their dependency score for the gene. Thus, each column indicates a rank. The line plots above the heatmaps indicate the representation (density) of each KRAS allele at each rank across the genes. d Hierarchically clustered heatmaps of the genes that demonstrated differential genetic dependency amongst cell lines of different KRAS alleles. Each column is a cell line labeled by its DepMap identifier and each row is a gene. e Examples of genes that demonstrated differential genetic dependency amongst cell lines of different KRAS alleles (t tests; FDR-adjusted p values). For the box plots, the box demarcations represent the 25th, 50th, and 75th percentiles, and the whiskers extend from the box to the largest and smallest data points at most 1.5 times the interquartile range away from the median. n = 23 biologically independent COAD cell lines. Source data are provided in the Source data file.
