Fig. 1: Genomics and genetic linkage analysis identify a gene for the thermosensory diguanylate cyclase (tdcA) in P. aeruginosa CF39S.
From: Bacterial cyclic diguanylate signaling networks sense temperature
a P. aeruginosa CF39S displays temperature-dependent rugose colony morphology on agar. b P. aeruginosa CF39S exhibits temperature-dependent biofilm formation in microplates. Datum points represent 16 technical replicates from each of three biological replicates, and lines and bars represent means and standard deviations, respectively. c Liquid chromatography tandem mass spectrometry (LC–MS/MS) measurements of cellular nucleotide pools revealed that P. aeruginosa CF39S displays temperature-dependent increases in cyclic diguanylate (c-di-GMP). Each datum point represents an independent biological replicate, and lines and bars represent means and standard deviations, respectively, for 3–5 independent biological replicates each. d Schematic illustrating the Tn7-like transposon in the P. aeruginosa CF39S chromosome encoding tdcA and its synteny to the E. coli AW1.7 locus of heat resistance (LHR). Annotations for the E. coli AW1.7 genome were taken from Mercer and colleagues44. Open reading frames are color coded by putative functions as illustrated in the legend: gray arrows denote genes of unknown function or functions other than those illustrated here; bluish green arrows denote genes for putative proteases or chaperones; yellow arrows denote genes for thioredoxins; orange arrow denotes a diguanylate cyclase; blue arrows denote genes encoding functions for transposition; and reddish purple segments denote inverted repeats. e Acquisition of tdcA confers thermal control of P. aeruginosa biofilm formation in microplates. Datum points represent six technical replicates from each of three biological replicates, and lines and bars represent means and standard deviations, respectively. In b, c, and e, tdcA− and tdcA+ strains are represented by sky blue and orange dots, respectively. Strains denoted tdcA− have the tdcA162ΔG allele. OD595nm denotes the optical density measured at a wavelength of 595 nm.
