Fig. 5: Design of assemblies with point group symmetry.
From: Design of multi-scale protein complexes by hierarchical building block fusion
a Tetrahedron design schematic. A HB and a C2 homo-oligomeric made from ankyrin repeat proteins were aligned to their respective tetrahedral symmetry axis (red), and connected via fusion to Ankyrin repeat monomers (blue) to generate the target architecture. b 3D reconstruction reveals a well fitting map of T_Wm-1606. c Icosahedral design schematic. Libraries of unverified cyclic fusion homo-dimers and trimers were aligned to the corresponding icosahedral symmetry axes. Using WORMS, fusions to DHRs split in the center that hold the two homo-oligomers in the orientations which generate icosahedral structures were identified. d Cryo-EM 3D reconstruction of I32_Wm-42 closely matches the designed model.
