Fig. 2: Proof-of-principle experiments of in vitro Cell-TALKING. | Nature Communications

Fig. 2: Proof-of-principle experiments of in vitro Cell-TALKING.

From: Cellular macromolecules-tethered DNA walking indexing to explore nanoenvironments of chromatin modifications

Fig. 2

a The one-to-two detection performance on DNA duplex substrates. b The one-to-three detection performance on DNA duplex substrates. c The one-to-many detection performance on DNA origami substrates. In a–c, five times of the experiment were repeated independently with similar results. d The distance-dependent detection performance on DNA origami substrates. The DNA duplex or DNA origami is modified on the surface of a coverglass. The scale bars of images are 25 and 20 μm in Fig. 1a, 10 μm in Fig. 1b, 20 nm and 5 μm in Fig. 1c, 15 μm in Fig. 1d, respectively. The BP and WP indicate the barcoding probe and walking probe, respectively. Five repeated experiments were performed for statistical analysis of spot count of randomly selected images, and the data are presented as mean values ± SD.

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