Fig. 3: Synthesis of ATP-γ-alkyne and labeling of DNA modifications. | Nature Communications

Fig. 3: Synthesis of ATP-γ-alkyne and labeling of DNA modifications.

From: Cellular macromolecules-tethered DNA walking indexing to explore nanoenvironments of chromatin modifications

Fig. 3

a MS characterization of ATP-γ-alkyne synthesis. Left panel, the mass spectrum of ATP (C10H15N5O13P3, [M + H]−: 505.98847, calculated; 505.98488, found); right panel, the mass spectrum of ATP-γ-alkyne (C13H18N6O12P3, [M + H]−: 543.02010, calculated; 543.01591, found). b Melting curve analysis of the cleavage products of 5hmU-contained dsDNA after labeled by 5-HMUDK and ATP-γ-alkyne. c MS characterization of 5fU reduction. Left panel, the mass spectrum of 5fU (C5H4N2O3, [M + H]−: 140.02274, calculated; 140.01658, found); right panel, the mass spectrum of reduction product 5hmU (C5H5N2O3, [M + H]−: 141.03057, calculated; 141.03095, found).

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