Fig. 6: Intra-tumour cellular phenotypic dynamics induced by mTOR inhibition. | Nature Communications

Fig. 6: Intra-tumour cellular phenotypic dynamics induced by mTOR inhibition.

From: Landscapes of cellular phenotypic diversity in breast cancer xenografts and their impact on drug response

Fig. 6: Intra-tumour cellular phenotypic dynamics induced by mTOR inhibition.The alternative text for this image may have been generated using AI.

a Schematic representation of the experimental setting. Single-cell suspensions of 4 PDTX models were treated with vistusertib (dosed from 0.1 to 10 μM) for 2 h and analysed using the breast cancer mass cytometry (BCMC) approach. b Heatmap of Earth Mover’s distance of OSA markers in the 4 PDTX models treated with vistusertib (dosed from 0.1 to 10 μM) compared with DMSO control. c tSNE plots in untreated and vistusertib-treated (10 μM) conditions. The intensity of each oncogenic signalling activation (OSA) subpanel marker is indicated by the colour gradient. Two model-specific responses (p-ERK induction in STG143_T1 and lack of inhibition of p-S6 in STG139_M1) are indicated by blue boxes. d Cell density plots of tSNE analysis from panel c. e Average Coefficient of Variation (CV) for OSA markers in untreated and treated (10 μM) conditions in the 4 tested PDTX models. Pairwise comparisons using two-sided t-test (p = 0.016). f Cell-cluster (CC) dynamics in each PDTX model upon vistusertib treatment (dosed from 0.1 to 10 μM). The model-specific cross-MC cell-classifier was applied to classify cells into each CC. Unclassified cells are indicated in grey.

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