Fig. 3: Metabolic analysis and apoptotic rate in CD8+ T cells from patients with SLE. | Nature Communications

Fig. 3: Metabolic analysis and apoptotic rate in CD8+ T cells from patients with SLE.

From: Type I interferons affect the metabolic fitness of CD8+ T cells from patients with systemic lupus erythematosus

Fig. 3

a Representative oxygen consumption rate (OCR) (left panels) and summary graphs (right panels) of sorted CD4+ and CD8+ T cells from controls, IFN-Neg and IFN-High SLE patients during a mitochondrial stress test. Spare respiratory capacity (SRC) was normalized to basal level of each individual. Oligomycin, carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP), and rotenone/antimycin A (R/A) were added to the cells as indicated. Each symbol represents an individual (HC n = 12; IFN-Neg n = 7; IFN-High n = 8). b PBMCs from IFN-Neg and IFN-High SLE patients and HC were rested in culture for 48 h followed by staining with Annexin V and propidium iodide (PI). Representative flow cytometry plots of CD3+ CD8+ gated cells and summary graphs. Each symbol represents an individual (HC n = 11; IFN-Neg n = 11; IFN-High n = 12) a, b Data presented as mean ± S.E.M. One-way ANOVA, only significant differences are indicated, HC healthy controls. Source data for this figure are provided as a Source Data file.

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