Fig. 1: H. pylori acquires T-repeat extension upstream of HPnc4160 to decrease its expression during infection in vivo.
From: A bacterial small RNA regulates the adaptation of Helicobacter pylori to the host environment
a Schematic showing experimental strategy. b RNA expression levels of ORFs or nearby genes of genome regions mutated in >50% of the strains recovered from gerbils, plotted against mutation rates. c DNA sequence around the HPnc4160 and T-repeat sequence of the strains recovered from gerbils. d–g Relative hpnc4160 expression in H. pylori strains recovered from gerbils (T repeat number of 14, n = 12; 16, n = 5; 17, n = 17; 18, n = 6) and genetically modified T-repeat lengths (n = 3) in RT-PCR (d, f) and Northern blot (e, g). Shown are the means ± s.d (d, f). Data were processed by Grubbs’ test for outliers. *P = 0.0379, **P = 0.0088, ****P < 0.0001 by Sidak’s multiple comparison tests (two sided) (d). Data are representative of two independent experiments (d–g). Source data are provided as a Source Data file.
