Fig. 9: Down-regulation of enolase-1 reduces bladder cancer cell proliferation. | Nature Communications

Fig. 9: Down-regulation of enolase-1 reduces bladder cancer cell proliferation.

From: Dominant role of CDKN2B/p15INK4B of 9p21.3 tumor suppressor hub in inhibition of cell-cycle and glycolysis

Fig. 9: Down-regulation of enolase-1 reduces bladder cancer cell proliferation.

a knockdown of enolase-1 by shRNA in UMUC3 cells leads to increased binding of p15 to CDK4 and CDK6 as evidenced by immunoprecipitation followed by western blotting. The two lanes represented two stable clones from each of scrambled control and enolase-1 shRNA. Source data are provided as a Source Data file. b, c Reduced enolase-1 was associated with reduced cell-cycle progression and increased cell proliferation. n = 3 and 4 for b and c, respectively, of biologically independent samples. Data are presented as mean values ± SD. Two-sided t-test was performed to compare the significance of the difference between the two groups (p15 versus p16). Experiments were repeated three times with similar results.

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