Fig. 3: Extract processing impacts vesicle size distributions and concentrations.
From: Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
a Illustration of extract processing conditions. Extracts were prepared in triplicate for each condition shown. b Nanoparticle Tracking Analysis (NTA) concentration analysis of vesicles in sonicated (blue) and homogenized (green) extracts. Asterisk indicates base case conditions for extract preparation. Data for (b) are presented as mean ± SD of n = 15 replicates (3 biologically independent extracts; each examined over 5, 1 min NTA measurements). c NTA particle size distribution of vesicles in sonicated (blue) and homogenized (green) S30 extracts. d NTA particle size distribution of sonicated (blue) and homogenized (green) S12 extracts. Data for (c, d) are presented as mean ± SEM of n = 15 replicates (3 biologically independent extracts; each examined over 5, 1 min NTA measurements). Source data for all panels are provided as a Source Data file.
