Fig. 8: Biochemical evidence that BioZ binds to its substrate glutaryl-CoA. | Nature Communications

Fig. 8: Biochemical evidence that BioZ binds to its substrate glutaryl-CoA.

From: Biochemical and structural characterization of the BioZ enzyme engaged in bacterial biotin synthesis pathway

Fig. 8

a Molecular docking of BioZ with glutaryl-CoA. b Structural snapshot of the glutaryl-CoA substrate-loading tunnel within BioZ enzyme. c Use of ITC to measure the binding of glutaryl-CoA to BioZ protein. A representative ITC result from three independent experiments is displayed. Thus, the stoichiometry values (N and Kd) are expressed in an average ± SD. The stoichiometry of glutaryl-CoA binding to BioZ enzyme is proposed to be 1:1, in that the molar ratio was measured to be around 0.8. The parameter of binding affinity (Kd) is 2.64 ± 0.23 μM.

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