Fig. 1: Increased number of UBC and expression of EOMES upon modelling of the BMI1^High;CHD7^Low signature in progenitor cells.

a, b Colours identify STOPFloxBmi1 (yellow), Chd7^f/+ (orange), Math1Cre;STOPFloxBmi1 (green) or Math1Cre;STOPFloxBmi1;Chd7^f/+ (pink) genotypes throughout the figure. EOMES IHC staining and quantification of UBC in the cerebellum at P7 (a) or P21 (b) developmental stages. a n = 2 biological independent animals per STOPFloxBmi1 and Math1Cre;STOPFloxBmi1 genotypes, n = 3 biological independent animals per Chd7^f/+ and Math1Cre;STOPFloxBmi1;Chd7^f/+ genotypes. b n = 3 biological independent animals per STOPFloxBmi1 and Chd7^f/+ genotypes, n = 4 biological independent animals per Math1Cre;STOPFloxBmi1;Chd7^f/+ genotype. One-way ANOVA. c NeuN IHC staining and quantification of GC in the cerebellum at P7. n = 2 biological independent animals per STOPFloxBmi1 and Math1Cre;STOPFloxBmi1 genotypes, n = 3 biological independent animals per Chd7^f/+genotype, n = 4 biological independent animals per Math1Cre;STOPFloxBmi1;Chd7^f/+ genotype, one-way ANOVA. d Heatmap showing z-scores of UBC marker expression in the human upper rhombic lip (light blue), cerebellum (yellow) and cerebellar cortex (green) from 8 to 17 pcw. e Heatmap showing relative expression of BMI1 and CHD7 in a collection of hNCS lines isolated from the cerebellum (yellow) or striatum (green) as compared to MB cell lines (pink). f qPCR analysis of EOMES expression level in hNSC upon CHD7 silencing (BMI1^High;CHD7^Low, purple) compared to control (CTRL, violet). n = 4 biologically independent experiments, two-tailed unpaired t test. g Cell proliferation assay of CDH7-silenced or control cells. n = 6 biologically independent experiments, two-way ANOVA. All graphs report mean ± SEM. P values: *P < 0.05, **P < 0.01 or ****P < 0.0001. Scale bars = 100 µm. Source data are provided as a Source Data file.