Fig. 3: LC-MS analysis of NgnO1-NgnO2, StmK, and NtfO1–NtfO2 catalyzed reaction.
From: A [6+4]-cycloaddition adduct is the biosynthetic intermediate in streptoseomycin biosynthesis
NgnO1-NgnO2 catalyzes a hydroxylation step on 6/7 or 5 when incubated with FMN and NADH; NgnO1 or NgnO2 alone has no effects on 6/7; NtfO1-NtfO2 has the same function with NgnO1-NgnO2; StmK catalyzes a ketoreduction step on the putative intermediate 12b; StmK can catalyze a reverse reaction using 11 as substrate and NADP+ as a cofactor.
