Fig. 3: Actin organization in dependence on bundler to actin ratio and membrane binding.

Actin is positioned close to the membrane regardless of membrane-anchoring. a Overview of conditions with varying actin concentration (2 µM and 6 µM), fascin to actin ratios (3.3:100–15:100) and with and without actin–membrane binding. Supplementary Movie 3 shows a 3D version of this figure panel. b Simulations for 6 µM conditions as in a using radius = 6 μm. c Average membrane proximity of actin signal for vesicles with 2 µM actin. Normalized range from −1 (all actin in the center of the vesicle) to +1 (all actin on the membrane). d Membrane proximity of actin signal for vesicles with 6 µM actin. Data in c and d are presented as mean values with individual data points and SEM. n = 10 vesicles for each condition (except n ≥ 3 for controls with 0% fascin).