Fig. 5: Actomyosin contraction leads to membrane deformations.
From: Reconstitution of contractile actomyosin rings in vesicles
a Time series of a contracting ring-like structure in a GUV. Same conditions as in Fig. 4c but with 0.1 µM myosin II. Only the midsection of the vesicle is shown, top and bottom are missing, but the yellow dotted lines in the first frame show the approximate position of the bundles (see also Supplementary Fig. 13). Cyan dotted lines in the following frames indicate the outline of the vesicle. Orange arrows indicate membrane deformations (vesicle constriction). The partially visible vesicle on the left can be seen to undergo a similar transition from a large actin network (t = 0) to myosin-constricted cluster (t = 60 min). Supplementary Movie 6 shows the same field of view. b Additional example of large-scale vesicle deformation through actomyosin contraction. Even though no furrow-like deformation was observed, the contraction of actin bundles still causes large-scale vesicle deformations (orange arrows).
