Fig. 6: Actin network governs vesicle shape in osmotically deflated vesicles.
From: Reconstitution of contractile actomyosin rings in vesicles
a Variety of vesicle shapes produced by different morphologies of encapsulated actin networks, some with stabilizing cortices and others with filopodia-like membrane protrusions. b Upon exposure to photodamage through increased laser power, cytoskeletal vesicles lose their stabilizing actin cortex, and often take on a flat and round shape. All three examples are shown in Supplementary Movie 7. c Vesicles with a stabilizing artificial actin cortex can be dried, frozen, and imaged using cryo-scanning electron microscopy.
