Fig. 6: Inducing LTD at excitatory inputs to NAc regulates high fat intake.
From: Input-specific modulation of murine nucleus accumbens differentially regulates hedonic feeding
a Schematic of viral infection and ferrule implant for aPVT input stimulation (left); experimental timeline (right). b Summary time course and representative traces of EPSC amplitude demonstrating optically induced depression of aPVT→ D1 MSN synapses (n = 4 cells). c Quantification of high fat intake following LTD induction (F3,51 = 3.07, P = 0.03, n = 11, 8 mice/group) in eYFP control (black) and ChR2 expressing (blue) mice. d Quantification of AMPAR/NMDAR ratio in D1 MSNs for aPVT inputs exposed to high fat without (red) and with (blue) LTD induction (t = 1.75, P = 0.04, n = 14,9 cells/group). (Data for high fat group (red) in d are the same as those shown in Fig. 5c.) e Schematic of viral infection and ferrule implant for PFC input stimulation (left); experimental timeline (right). f Quantification of high fat intake following LTD induction (F1,15 = 4.57, P = 0.04, n = 8, 9 mice/group) in eYFP control (black) and ChR2 expressing (blue) mice. g Schematic of implant for electrical stimulation (left); experimental timeline (right). h Quantification of high fat intake following LTD induction (F3,24 = 4.59, P = 0.03, n = 9 mice). Gray bars signify electrical stimulation. Data are ±s.e.m. *P < 0.05, b Student’s t test two-tailed, c, f repeated measure two-way ANOVA with Sidak’s multiple comparison test, d Student’s t test one-tailed, h one-way ANOVA with Holm-Sidak multiple comparison test. Source data are provided as a Source Data file.
