Fig. 1: Cortical microtubules in 26S proteasome mutant plants are insensitive to ABA-induced disassembly.
From: The E3 ligase MREL57 modulates microtubule stability and stomatal closure in response to ABA
a Cortical microtubules in guard cells from WT and rpn1a-4 seedlings treated with 5 μM oryzalin for the indicated times. Scale bar = 10 μm. b The graph shows the densities of microtubules in a. Data represent mean ± standard deviation (SD) values from three independent experiments with a minimum of 15 cells each. c Cortical microtubules in guard cells from WT and rpn1a-4 seedlings treated with 10 μM ABA for 40 min. Scale bar = 10 μm. d The graph shows the densities of microtubules in c. Data represent mean ± standard deviation (SD) values from three independent experiments with a minimum of 15 cells each. e Detached rosette leaves from WT, rpn1a-4, and rpn10-1 were incubated in opening buffer for 2 h and then treated with 10 μM ABA plus 0 or 5 μM oryzalin for 2 h. The box and whiskers plots represent minimum and maximum values. The line in the box indicates the median value and the boundaries demonstrate the 25th percentile (upper) and the 75th percentile (lower). Different letters represent significant differences at p < 0.01 (one-way ANOVA). The experiment was repeated three times as different biological replicates with a minimum of 100 stomatal pores.
