Fig. 5: Antibiotic treatment is associated with changes in lipid composition.
From: Cytoplasmic condensation induced by membrane damage is associated with antibiotic lethality
a Representative thin-layer chromatography image for lysates of control and antibiotic-treated cell cultures. Cells were harvested after various treatment times as shown, and antibiotic concentrations used were 10× MIC. For comparison, results for cells treated with 10 mM hydrogen peroxide and 10× MIC ampicillin are shown. Results are representative of two biological replicates. Abbreviations: CL, cardiolipin; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; FFA, free fatty acids; DAG, diacylglycerol; OA, oleic acid. b, c Relative FFA levels (b) and CL/PE ratios (c) across treatment conditions, relative to values for untreated cells at each treatment time. Data are from two biological replicates (black points), and bars indicate averages.
