Fig. 3: Effects of mg-Nrp1 cko on OPC proliferation in the white matter. | Nature Communications

Fig. 3: Effects of mg-Nrp1 cko on OPC proliferation in the white matter.

From: Microglial neuropilin-1 promotes oligodendrocyte expansion during development and remyelination by trans-activating platelet-derived growth factor receptor

Fig. 3

A Schematic of generating microglia-specific Nrp1 knock out (mg-Nrp1-cko) and the heterozygous control (mg-Nrp1-cont). B Schematic of tissue analysis at P5 and P14 after cre induction at P2-3. C, D. 3D images of P5 corpus callosum labeled for Nrp1 (red), EYFP (green), PDGFRα (light blue), and EdU (pink). Scale, 20 μm. Arrows, examples of EdU+ PDGFRα+ cells in contact with EYFP + microglia. E Quantification of proliferation of OPCs in mg-Nrp1-cont (black) and cko (blue). E1 Total EdU+ OPC density in P5 corpus callosum. Unpaired Student’s t-test, n = 5, t = 7.171, df = 8. E2 The density of EdU+ OPCs in cont or cko with (circles) or without (triangles) contact with microglia. Two-way ANOVA, Sidak’s multiple comparisons test, F(1, 16) = 53.41, p < 0.0001, n = 5. F Proportion of PDGFRα+ OPCs that were in contact with EYFP + microglia. Unpaired Student’s t-test, n = 5, t = 3.940, df = 8. G Proportion of OPCs that were EdU+ in P5 cerebellum, P5 corpus callosum, and P14 corpus callosum. Two-way ANOVA, Sidak’s multiple comparisons test. F(1, 13) = 32.44, p < 0.0001, n = 3–4. H Quantification of PDGFRα+ OPC density in P5 and P14 corpus callosum. Two-way ANOVA, Sidak’s multiple comparisons test. F(1, 12) = 19.07, p = 0.0009, n = 5 (P5), n = 3 (P14). I Quantification of MBP immunofluorescence intensity in P14 corpus callosum of cont and cko mice. n = 3, t = 0.1896, df = 4. Black symbols, mg-Nrp1-cont; blue symbols, mg-Nrp1-cko in E–I. Source data are provided as a Source Data file.

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