Fig. 5: The extent of oligodendrocyte regeneration and myelin repair in mg-Nrp1-cko. | Nature Communications

Fig. 5: The extent of oligodendrocyte regeneration and myelin repair in mg-Nrp1-cko.

From: Microglial neuropilin-1 promotes oligodendrocyte expansion during development and remyelination by trans-activating platelet-derived growth factor receptor

Fig. 5

A, B Labeling mg-Nrp1-cont (L) and cko (M) for CC1 (red), DAPI (blue), and EYFP (green) at 14 dpl. Arrowheads, CC1+ cells. Scale, 25 µm. C Quantification of OL density in cont and cko lesions at 14 dpl. Student’s t-test, unpaired, two-tailed, n = 5, t = 4.753, df = 8. D, E Labeling mg-Nrp1-cont (O) and cko (P) for MBP (blue) and non-phosphorylated neurofilaments (non-P-NF, red) using smi-32 antibody to identify axons that have not yet undergone complete remyelination. Dotted lines indicate the extent of partial or complete demyelination. Scale, 50 μm. LV, lateral ventricle. F Quantification of demyelinated area in cont and cko lesions at 14 dpl. Student’s t-test, unpaired, two-tailed, n = 5, t = 3.490, df = 8. G, H Labeling for MBP (blue) and non-phosphorylated neurofilaments (non-P NF, red) in the lesioned corpus callosum of mg-Nrp1-cont (G) and cko (H) mice at 28 dpl. I Quantification of MBP immunofluorescence in the lesioned corpus callosum of mg-Nrp1-cont and cko mice at 28 dpl. Student’s t-test, unpaired, two-tailed, n = 5, t = 4.031, df = 8. Black circles, mg-Nrp1-cont; blue squares, mg-Nrp1-cko in C, F, and I. J, K Electron microscopic images of cross-sections of LPC-lesioned corpus callosum at 28 dpl. J mg-Nrp1-cont (fl/+) showing sheaths of various thickness (arrows), but most axons appear fully myelinated. K mg-Nr1p-cko (fl/fl) showing more axons without myelin sheaths (arrowheads), axons with thin myelin (arrow), and abundant swollen glial cell processes (*). Scale bars 1 μm. Source data are provided as a Source Data file.

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