Fig. 6: TIM4 is required for tumor rejection induced by immunogenic chemotherapy. | Nature Communications

Fig. 6: TIM4 is required for tumor rejection induced by immunogenic chemotherapy.

From: TIM4 expression by dendritic cells mediates uptake of tumor-associated antigens and anti-tumor responses

Fig. 6

a Scheme of experimental design. Mice were challenged with KP cells and left untreated or treated with oxa/cycl and anti-TIM4 or isotype as depicted in the scheme. b, c Analysis of the CD8/CD4 T cell ratio in the MLNs (b), Untreated n = 10, Oxa/Cycl+Isotype n = 9, Oxa/Cycl+αTIM4 n = 9; and in the lungs (c), Untreated n = 10, Oxa/Cycl+Isotype n = 10, Oxa/Cycl+αTIM4 n = 9; of mice 2 weeks after the last treatment. Data are from two independent experiments. d Effect of TIM4 blockade on the efficacy of the immunogenic chemotherapy. Tumor burden was assessed at day 28 by calculating the area covered by tumor nodules on four consecutive sections of three lobes/animal and expressed as a function of the total lobe area. Results are from untreated n = 13, Isotype+Oxa/Cycl n = 14 and Oxa/Cycl+αTIM4 n = 14 in two independent experiments. e WT and Batf3−/− mice were challenged with KP cells and treated with oxa/cycl or left untreated. Treatment efficacy is expressed as the percentage of reduction in tumor area over not treated control calculated in 14 WT and 4 Batf3−/− mice. All data are expressed as mean ± SEM. Significance was determined by one-way ANOVA followed by Tukey’s post-test for b–d and two-tailed Mann–Whitney test in e. Source data are provided as a Source Data file.

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