Fig. 2: F4/80⁺ macrophages accumulate on fibrin clots in the damaged peritoneum.

a Representative tiling image of immunofluorescence staining for the ischemic button on day 1 post-surgery. Cross-sections were stained for PDPN and F4/80. The inserted images present the higher magnification of the surface area of the ischemic button and the remote area from the ischemic button. n = 3 independent ischemic buttons. Scale bar, 1 mm. b Whole-mount immunohistostaining of the ischemic button surface. n = 3 independent ischemic buttons. Scale bars, 100 μm. c, d Representative immunofluorescence images and quantification of ischemic buttons created in the mouse peritoneal membrane. Cross-sections were stained for PDPN, F4/80, and fibrin. The naive peritoneum was examined as “Day 0”. The graph shows the time course of the cover ratio of the ischemic button surface by PDPN⁺ mesothelial cells or F4/80⁺ macrophages. n = 6 (Day 0), 5 (Day 1), 7 (Day 3), and 6 (Day 5) mice. Data represent the mean ± SEM. Scale bar, 100 μm. e Representative immunofluorescence images of the adhesion formed between the ischemic button and surrounding tissue (intestine) on day 3 post-surgery. n = 3 independent experiments. Scale bars, 200 μm.