Fig. 4: RelA-mediated Hfq assembly requires interaction with GGAGA sequence (western using α Hfq antibody).
From: RNA binding of Hfq monomers promotes RelA-mediated hexamerization in a limiting Hfq environment
a, c In the absence of RelA, RNA has no effect on Hfq multimerization. Reactions of Hfq incubated without or with RNA (100 nM) at 22 °C for 10 min were UV crosslinked followed by protein crosslinking with 0.2% of glutaraldehyde (Time in min indicates duration of protein crosslinking). The proteins separated in 4–20% MOPS gradient gels were detected using α Hfq antibody. b, d RelA induces Hfq multimerization when presented with RNA carrying GGAGA. Reactions of Hfq incubated with RelA, without or with RNA including RyhB (90 nt), RyhBm (90 nt) sodA (98 nt), and sodA-ΔSD (47 nt) were treated as in a. Asterisk indicates the formation of new Hfq multimers detected using wild type RNAs in the presence of RelA. Source data provided as a source data file.
