Fig. 3: CRISPR/Cas9 targeting of KCNJ11 protein-coding sequence in primary human islets.
From: CRISPR-based genome editing in primary human pancreatic islet cells
A, B Human pseudoislets 5 days post-infection with CRISPR-KCNJ11 lentiviruses (A) bright field, (B) blue light (488 nm), scale bar: 500 μm (n = 3 independent donors). C Schematics of KCNJ11 sequence, showing the sgRNA sequence (sgRNA_K, red). Arrows indicate primers used for PCR. D Percentage of indel-modified sequences detected by TIDE algorithm (n = 3 independent donors; P = 0.0225). E qRT-PCR of GFP+ cells, CRISPR-KCNJ11 (red), CRISPR-Control (Black) (n = 5 independent donors; P = 0.0175). F Patch clamping in single β-cells: measurement of KATP currents in CRISPR-KCNJ11 (n = 19 cells, 3 replicates) and CRISPR-Control (n = 25, 3 replicates) (P < 0.0001). Data are presented as mean values ± SD for (D–E) and mean values ± SEM for (F). Two-tailed t tests were used to generate P-values. *P < 0.05, **** P < 0.0001. Source data are provided as a Source Data file.
