Fig. 3: Cell death triggered by SGP1 requires BAK1 but is not affected after treatment with PI-PLC or heat in N. benthamiana.

a–c Cell death triggered by SGP1 requires BAK1. a SGP1-triggered cell death in TRV-silenced N. benthamiana. Representative photographs were taken at 5 days post-infiltration. Data were obtained from 3 independent experiments, each of which used 10 VIGS-silenced plants per line (Supplementary Data 3). The plus symbol (+) indicates a positive cell death phenotype; minus symbol (−) indicates the absence of a clear cell death phenotype. b Western blot analysis of proteins transiently expressed in VIGS-silenced N. benthamiana leaves. The red asterisks (*) indicate the predicted protein sizes. c qRT-PCR quantification of relative transcript levels of BAK1 in VIGS-silenced N. benthamiana leaves, normalized to those in TRV:EV lines, using the EF1α gene as an internal reference. Means and standard deviations obtained from three biological replicates. (**P < 0.01, two-sided t-test). d Cell death activity of SGP1rec in the presence or absence of PI-PLC, with MBP as a control. Data represent the results of three independent experiments in each of which one leaf was sampled and infiltrated from each of three plants per treatment (Supplementary Data 3). e Cell death response induced by heat-treated SGP1rec in N. benthamiana leaves. SGP1rec was treated under 100 °C for 10 min. Three independent experiments were performed (Supplementary Data 3), in each of which ten leaves were sampled per treatment. The plus symbol (+) indicates a positive cell death phenotype; minus symbol (−) indicates the absence of a clear cell death phenotype.