Fig. 5: A 22 amino acid peptide (SNP22) of SGP1 is sufficient to induce ROS production in N. benthamiana.
a Schematic representation of synthetic peptides (30-mer peptides, a–r) covering wild-type SGP1. b ROS production dynamics induced by 1 μM peptides of SNP22 and SNP22W44A in N. benthamiana leaf discs. 100 nM flg22 and buffer served as positive and negative controls, respectively. ROS production was measured with a luminol-based assay. Leaf discs from 3- to 4-week-old N. benthamiana plants were incubated with the indicated peptides, and the luminescence was expressed as relative light units (RLU). Values represent means ± SEM of three leaf disks, each sampled from a different plant and tested in parallel. c Dose–response curves of total ROS production induced by SNP22, SNP21, SNP20, SNP22I25A, or SNP22W44A peptides in N. benthamiana leaf discs. Values represent the means ± SEM of three independent experiments, each of which used three leaf disks per treatment. d Summary of EC50 values determined from dose–response curves based on total ROS production. Peptide names and sequences are indicated on the left. EC50 values of each peptide are shown on a logarithmic scale on the right. Values represent the means ± SEM of three independent experiments, with three leaf disks per treatment used in each experiment. No activity could be detected with peptides denoted by red triangles at 10 μM. Vm, KUI59516 (Valsa mali); Bc, Bcin01p05310.2 (Botrytis cinerea); Ss, EDO01950 (Sclerotinia sclerotiorum); Cp, CCE31100 (Claviceps purpurea); Fv, FVEG_00876T0 (Fusarium verticillioides); Fs, NechaP98847 (Fusarium solani); Vd, VDAG_JR2_Chr3g03370a-00001 (Verticillium dahliae); Mo, MGG_04599T0 (Magnaporthe oryzae).
