Fig. 1: Partial male sterility induced by callose accumulation in sperm cells. | Nature Communications

Fig. 1: Partial male sterility induced by callose accumulation in sperm cells.

From: Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex

Fig. 1

a, b Aniline blue staining of mature pollen from wild-type Col-0 plants (Col-0, a) or hemizygous pHTR10:cals3m plants (SC-cal, b). Arrows in (b) indicate callose deposition in sperm cells. Images are representative of three (a) or six (b) observations. c, d Transmission of SC-cal transgene from female gametophytes (c) or male gametophytes (d) analyzed by hygromycin resistance of T1 plants obtained following reciprocal crosses between the SC-cal transgenic lines and wild-type Col-0 plants. Line 2 shows higher hygromycin resistance in (c) possibly due to the multiple insertions of the SC-cal. Numbers above the bars indicate seeds analyzed. e, f Developing seeds in Col-0 (e) or an SC-cal transgenic line (f). Images in (e) or (f) show representative of two pistils analyzed. Asterisks in (f) represent undeveloped seeds. Scale bars: 20 μm in (b, c); 1 mm in (e, f).

Back to article page