Fig. 8: Guidance of pollen tubes containing immotile vegetative nucleus and sperm cells. | Nature Communications

Fig. 8: Guidance of pollen tubes containing immotile vegetative nucleus and sperm cells.

From: Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex

Fig. 8

a Schematic drawing of the single pollen-tube guidance assay. b–d Representative images of ovular-targeting pollen tubes visualized by aniline blue staining after the single pollen-tube guidance assay. Pollen tubes from wild-type Col-0 pollen (Col-0, b). Pollen tubes from tdTomato-negative pollen (wit1/2 SC-cal − RHT −, c) or tdTomato-positive pollen (wit1/2 SC-cal + RHT +, d) from a wit1 wit2 mutant line hemizygous for genetically linked pHTR10:cals3m and pRPS5A:H2B-tdTomato (identical to the wit1/2 SC-cal RHT line shown in Fig. 5). The right panels in (d) represent the RHT + pollen tube on the stigma; magnification of the dashed box in the upper-right is shown in the lower-right. Scale bar: 100 μm. Images are representative of 22 (b), 24 (c), or 22 (d) independent assays showing similar patterns. e Percentage of pollen tubes that emerged from the cut style. f Percentages of pollen tubes that entered the micropyle in the ovules. Statistical test: Bonferroni-adjusted significance test was performed after one-way ANOVA. Asterisk indicates significance (P = 0.0438).

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