Fig. 5: BiRN predicts therapeutic efficacy of PDPA-PTX nanoparticle. | Nature Communications

Fig. 5: BiRN predicts therapeutic efficacy of PDPA-PTX nanoparticle.

From: Quantitative imaging of intracellular nanoparticle exposure enables prediction of nanotherapeutic efficacy

Fig. 5: BiRN predicts therapeutic efficacy of PDPA-PTX nanoparticle.The alternative text for this image may have been generated using AI.

a The design of PDPA-PTX and schematic of internalisation and PTX release of PDPA-PTX in cells. b TEM images of PDPA-PTX at pH 7.4 and 5.4. Polymer concentration, 1 mg mL−1, scale bar, 100 nm. c In vitro PTX release profiles of PDPA-PTX in different conditions. Mean ± s.d. (n = 3 biologically independent experiments). d Confocal images of 4T1 cells treated with BiRN (100 μg mL−1) with or without dynasore. e Cytotoxicity of free PTX and PDPA-PTX micelle. Mean ± s.d. (n = 6 biologically independent experiments). f Correlation between intracellular nanoparticle exposure and intratumoural levels of released PTX at 48 h post-injection of BiRN and PDPA-PTX (PTX, 10 mg kg−1). R and P values were derived using a simple linear regression model. The error band in red shows the 95% confidence intervals of the fitted line by two-sided Student t-test analysis. g Experimental design for using BiRN to predict PDPA-PTX response in 4T1 orthotopic tumour model. h Fluorescence images of 4T1 tumour-bearing mice were captured at 24 h post-injection of BiRN (20 mg kg−1) (n = 24 biologically independent mice). i Intracellular nanoparticle level of 4T1 orthotopic tumour-bearing mice in h. Mean ± s.d. (n = 24 biologically independent mice) j Tumour progression in 4T1 orthotopic tumours ranked according to low and high internalisation. ***P = 0.0005 at day 2, *P = 0.0123 at day 4, *P = 0.0411 at day 6, ****P < 0.0001 at day 6 and day 8, Two-way analysis of variance (ANOVA). Mean ± s.d. (n = 9 mice for PBS group; n = 12 for other groups). k Correlation between intracellular nanoparticle level and 4T1 tumour progression presented as tumour inhibition percentage (n = 24 biologically independent mice). R and P values were derived using a simple linear regression model. The error band in red shows the 95% confidence intervals of the fitted line by two-sided Student t-test analysis. l Lung metastases imaged by in vivo BLI analysis. m Quantitative analysis of mice thoracic cavity signal in l (n = 12 biologically independent mice).

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