Fig. 2: Bif3 gene identification.
From: Structural variation at the maize WUSCHEL1 locus alters stem cell organization in inflorescences
a Map-based cloning of Bif3 (in parenthesis, number of recombinants R/total chromosomes). b RNA-seq analysis of genes (G) in the mapping window indicates that ZmWUS1 is differentially expressed (fold-change = 13.6); two-tailed Student’s t-test ****p < 0.0001). n+/+ = 3, nBif3/Bif3 = 4 (n pools of three ear samples). Plotted data represent mean ± standard deviation. c qRT-PCR of ZmWUS1 in immature ears; two-tailed Student’s t-test **p < 0.01; n+/+ = 4, n+/Bif3 = 3, nBif3/Bif3 = 3 (n pools of three ear samples). Plotted data represent mean ± standard deviation. d Structure of the tandem-duplicated ZmWUS1 locus in the Bif3 mutant. A 16 kb fragment containing ZmWUS1 was duplicated and inserted in the first intron of GRMZM2G046968. Triangles represent transposon insertions from different families.
