Fig. 1: PRDM3 interacts with PAX8.
From: PAX8 and MECOM are interaction partners driving ovarian cancer
A BioID-MS results from IGROV-1-PAX8-BioID-T2A-mCherry cells. Blue dots represent proteins significantly enriched (P value <0.01 and Log FC >1). Red dot represents PAX8 and orange dot represents MECOM. B List of transcription factors enriched in PAX8-BioID IP-MS experiment. C Western blot from BioID-WB experiments in two different IGROV-1-PAX8-BioID-T2A-mCherry clones. The picture displays one representative image out of three independent experiments. D Endogenous co-immunoprecipitation between PAX8 and MECOM variants in MFE-319 and IGROV-1 cells. The picture displays one representative image out of three independent experiments. E Co-immunoprecipitation of ectopically expressed PAX8-HA and PRDM3 in HEK293 cells. The picture displays one representative image out of five independent experiments. F NanoBit assay in HEK293A cells transfected with PAX8-LgBit (Lg) and PRDM3-SmBit (Sm). HNF1B and PCBD1 are an unrelated pair used as positive and specificity controls. RLU relative luminescence unit. Data are presented as mean values ± SD from three biological replicates. Source data for Western blots and interaction measurements are provided as a Source Data file.
