Fig. 3: HF-HI impairs chronic cognitive function.

a Schematic of the experimental design for this figure. Mice were subject to sham or HF-HI before cognitive assessment at 1 month after the final impact. b T-maze cognitive assessment at 1 month showed decreased working memory in HF-HI mice (n = 13 sham, n = 18 HF-HI, *P = 0.0356, unpaired, two-tailed t test). c Latency to platform during the acquisition phase of the Barnes maze shows decreased learning in HF-HI mice. Data from two trials per day on 4 consecutive days (n = 20 per group, *P = 0.04, two-way RM ANOVA with Bonferroni post hoc test). d Heatmaps detailing group time spent in Barnes maze during the probe trial at 72 h post acquisition. e Number of entries into escape target area during the 72 h delay probe trial is reduced in HF-HI mice (n = 20 per group, *P = 0.029, unpaired, two-tailed t test). f Time spent in Barnes maze escape quadrant is reduced in HF-HI mice during the 72 h delay probe trial (n = 20/group, **P = 0.009, two-way ANOVA with Bonferroni post hoc test). g Speed and h distance traveled is similar between groups (n = 20 per group). i Latency to platform is increased in HF-HI mice during the acquisition phase of the Morris Water maze (MWM). Data from two trials per day on 4 consecutive days (n = 21/group, *P = 0.016, two-way RM ANOVA with Bonferroni post hoc test). j Heatmaps detailing group time spent in MWM during the probe trial. k Number of platform crossings is reduced in HF-HI mice during the 72 h delay probe trial (n = 21 per group, **P = 0.006, unpaired, two-tailed t test). l Number of entries into the target quadrant was reduced in HF-Hi mice during the 72 h delay probe trial (n = 21 per group, ****P < 0.0001, two-way ANOVA with Bonferroni post hoc test). m Time spent in MWM quadrants showed decreased time in the target zone for HF-HI mice during 72 h delay post acquisition (n = 21 per group, *P = 0.048, two-way ANOVA with Bonferroni post hoc test). Data are shown as mean ± SEM.