Fig. 1: Neurexins are required for intact functional organization of GABAB-receptors at the calyx of Held.
From: Neurexins regulate presynaptic GABAB-receptors at central synapses
a The diagram of the calyx of Held synapse. b Strategy for selective deletions of all neurexins at the calyx of Held by crossing PV-Cre mice with triple Nrxn123 cKO mice (Chen et al.33, Luo et al.34). c Representative traces of EPSC before and after application of 20 µM SKF-97541 (SKF), a potent and selective GABAB-receptor agonist, recorded in acute slices from littermate control and neurexin123 TKO mice at P12–P14. The normalized EPSCs before and after SKF are shown in inset. d Summary graphs of EPSC1 amplitudes before and after SKF for control and Nrxn123 TKO mice. P = 2.3E−7, P = 0.072, paired two-sided t-test. P = 0.0062, unpaired two-sided t-test. e Summary graphs of EPSC1 remaining unblocked by SKF application. P = 1.79E−7, unpaired two-sided t-test. f Summary graphs of the paired-pulse ratio (PPR) before and after SKF in control and Nrxn123 TKO mice. P = 2.81E−5, P = 0.09, paired two-sided t-test. P = 0.0038, unpaired two-sided t-test. Data are means ± SEM. Number of cells (from at least three mice per group) analyzed are indicated in the bars (d–f); Statistical differences were assessed by Student’s t-test. (*P < 0.05; **P < 0.01; ***P < 0.001). Source data are provided as a Source Data file.
