Fig. 2: Neurexins are required for the modulation of presynaptic Ca²⁺-channels by GABA_B-receptors at the calyx terminal.

a Example traces of the presynaptic Ca²⁺ currents induced by a 50 ms step depolarization (from −60 mV to +40 mV in 10 mV increments) before and after application of 10 µM SKF, recorded from the calyx terminals in acute slices from littermate control and Nrxn1/2/3 TKO mice at P12–P14. b Summary graphs of the current-voltage relationship of presynaptic Ca²⁺ currents before (thick) and after (thin) activating GBAB_B-receptors by SKF. c Summary graphs of the current density measured by depolarization to +10 mV before and after SKF. P = 1.8E−6, P = 0.039, paired two-sided t-test. P = 0.61, unpaired two-sided t-test. d Summary graphs of Ca²⁺ current inhibition by SKF. P = 0.0041, unpaired two-sided t-test. Data are means ± SEM. Number of cells (from at least three mice per group) analyzed are indicated in the bars (c, d); Statistical differences were assessed by Student’s t-test. (**P < 0.01). Source data are provided as a Source Data file.