Fig. 7: Deletion of neurexins impairs the function of GABAB-receptors at the inhibitory synapse between basket cells and Purkinje cells in the cerebellum.
From: Neurexins regulate presynaptic GABAB-receptors at central synapses
a Schematic of cerebellum circuits and experimental approach for stimulating basket cell (BC)-Purkinje cell (PC) inhibitory synapse. b Example traces of paired-pulse IPSCs before and after addition of GABAB-receptor agonist SKF, recorded in acute cerebellar slices from littermate control and Nrxn123 TKO mice P35-49. The normalized IPSCs before and after SKF are shown in inset. c Summary graphs of IPSC1 amplitudes before and after SKF for control and Nrxn123 TKO synapses. P = 0.0016, P = 0.051, paired two-sided t-test. P = 0.0389, unpaired two-sided t-test. d Summary graphs of IPSC1 amplitude remaining unblocked by SKF for control and Nrxn123 TKO mice. P = 0.0028; unpaired two-sided t-test. e Summary graphs of the PPR before and after SKF in control and Nrxn123 TKO mice. P = 0.000101, P = 0.097, paired two-sided t-test. P = 0.762, unpaired two-sided t-test. f Summary graphs of the CV of IPSC1 amplitude before and after SKF in control and Nrxn123 TKO mice. P = 0.00073, P = 0.066, paired two-sided t-test. P = 0.348, unpaired two-sided t-test. g Summary graphs of IPSC1 rise time (left) and decay time constant (right). P = 0.000953, P = 0.977; unpaired two-sided t-test. Data are means ± SEM. Number of cells (from at least three mice per group) analyzed are indicated in the bars (c–g); Statistical differences were assessed by Student’s t-test. (*P < 0.05; **P < 0.01; ***P < 0.001). Source data are provided as a Source Data file.
