Fig. 6: Lrig1 overexpression in NSCs induces cell cycle exit.
From: LRIG1 is a gatekeeper to exit from quiescence in adult neural stem cells
a IHC for FLAG (green), and GFAP (red). Detection of EdU (magenta) and nuclear counterstaining in blue (DAPI) with NSC overexpressing Lrig1 in different concentrations of mitogens (10, 1, and 0.1 ng/ml of both EGF/FGF). b Quantification of EdU positive cells after 2 h of pulse in the FLAG + and FLAG- populations. c IHC for CD9 (red) and FLAG (green). Detection of EdU in magenta and nuclear counterstaining with DAPI (blue). d IHC of FLAG (green), active CASPASE-3 (red). Detection of EdU (magenta) and nuclear counterstaining with DAPI (blue). Cells analyzed 48 and 96 h after transfection. e Quantification of EdU positive cells in the FLAG + and FLAG- population after 48 h and 96 h of transfection. f Quantification of active CASPASE-3 after 48 h and 96 h of transfection in FLAG + and FLAG- populations (n = 3 independent transfections, minimal number of 500 cells per condition). Scale bar in (a) is 50 μm, (c) is 10 μm and (d) is 30 μm. Data are shown as mean ± SEM of the indicated number of the experiments (n). Source data are provided as Source Data File.
