Fig. 2: African spiny mice exhibit an optimal range of tissue stiffness for placode formation. | Nature Communications

Fig. 2: African spiny mice exhibit an optimal range of tissue stiffness for placode formation.

From: Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice

Fig. 2: African spiny mice exhibit an optimal range of tissue stiffness for placode formation.

a A spiny mouse on PWD28. b K15 wholemount immunostaining of a PWD28 spiny mouse wound. Red line: border of the wound bed. c Schematic diagram of hair follicles (blue dot) in b. d The stiffness heatmap overlaying the PWD14 spiny mouse wound. Colorimetric unit: kPa. e H&E histology and stiffness heatmap of PWD14 and f PWD21 wounds. The color and number indicate the stiffness of the wound at respective location. Unit: kPa. (e1–e3) enlarged images of regions 1, 2, and 3 from e. g H&E of the PWD28 wound. Yellow arrows indicate the formation of hair placode. h SHG of PWD14 and PWD21 spiny mouse wounds. Red line: wound border. i, IHC of collagen I and collagen III in spiny mouse at different post-wound time. j Blebbistatin treatment significantly reduced the resultant number of hair neogenesis. n = 5 biologically independent animals. Data are presented as mean values ± SEM. p < 0.0001, unpaired two-sided t-test. k Graph indicating the respective wound stiffness of the specific wound location and time in the laboratory and spiny mice. n = 10 regions examined over 5 biologically independent animals per location per condition. Data are presented as mean values ± SD. ###p < 0.0001 when compared between Mus PWD14 and Acomys PWD14; *p = 0.0482 when compared between Mus PWD14 and Mus/Bleb PWD14 center, p = 0.0218 when compared between Mus PWD14 and Mus/Bleb PWD14 at the right of wound center, p = 0.0441 between Acomys PWD14 and Acomys PWD21 at the left of wound center, p = 0.0381 when compared between Acomys PWD14 and Acomys/Bleb PWD14 wound margin right, p = 0.0421 at center-right, **p = 0.0089; ***p < 0.0001. One-way ANOVA, Tukey test. l Illustration of morphogenetic field in PWD14 and PWD21 spiny mouse wound bed. Red: wound border. Green: morphogenetic field. ###: p < 0.005. Ctl: control; Bleb: Blebbistatin; Lt: left; Rt: right; Morph: morphogenetic. The images in a–g, i represent five out of five experiments performed. The images in h represent 4 out of 4 experiments performed.

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