Fig. 4: Twist1 is also expressed and can modulate the outcome of WIHN in spiny mice. | Nature Communications

Fig. 4: Twist1 is also expressed and can modulate the outcome of WIHN in spiny mice.

From: Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice

Fig. 4: Twist1 is also expressed and can modulate the outcome of WIHN in spiny mice.

a Gene expression heatmap of spiny mouse epidermis at different post-wound days. b Proliferation of epithelial cells and cell movement associated genes in the spiny mouse wound epidermis. c Twist1 related transcription factors in the spiny mouse wound dermis. d ECM remodeling in the spiny mouse wound dermis. e IHC of Twist1, Zeb2, MMP9, E-cad and P-cad in PWD14 the regenerating hair placodes of the spiny mice. White arrows point to the downregulation of E-cad at the tip of hair placode. f The effects of Twist1 inhibitor Harmine and pan-MMP inhibitor GM6001 treatment on hair neogenesis in spiny mouse. Red: wound border; yellow arrow: hair follicles. Observed on PWD35. g Dot graph of the number of regenerated hair follicles (HF) observed in spiny mouse wounds under different treatments. n = 6 biologically independent animals. Data are presented as mean values ± SEM. *p = 0.0265; **p = 0.0027; unpaired two-sided t-test. Ctl: control. The images in f represent six out of six experiments performed. The images in e represent 4 out of 4 experiments performed.

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