Fig. 4: The failure of Oo-Mvi formation in Oo-Rdx−/− females led to abnormal folliculogenesis.
From: Oocyte-derived microvilli control female fertility by optimizing ovarian follicle selection in mice
a Histological analysis of the ovarian morphological changes in Oo-Rdx−/− females, showing a decrease in ovarian size and an increase in follicle death (arrows) in the mutant ovaries. b Follicle counting confirmed a fast follicle loss in Oo-Rdx−/− ovaries. P-value: 0.98 (PD5, n = 3), 0.0015 (PD35, n = 5), 0.0008 (2 M, n = 3), 0.03 (8 M, n = 3). c, d Tracing the growth of oocytes in cultured ovarian pieces of Oo-Rdx+/+;mTmG and Oo-Rdx−/−;mTmG (c). A significant retardation of oocyte growth in Oo-Rdx−/−;mTmG (n = 11) ovaries compared to that in Oo-Rdx+/+;mTmG (n = 11) ovaries (d). P-value: 0.015 (day 3), 0.0002 (day 7). e, f Tracing the growth of ovarian follicles from Oo-Rdx−/− and Oo-Rdx+/+ ovaries in vitro (e), showing significant retardation of the increase in follicle diameter in Oo-Rdx−/− mice (n = 9, red) compared to that in controls (n = 6, black). P-value: 0.0057 (day 4), 0.014 (day 8) (f). Representative images are shown. Data are presented as the mean ± SD. Data were analyzed by two-tailed unpaired Student’s t-test and ***P < 0.001, **P < 0.01, *P < 0.05. Scale bars: 500 μm (black) and 100 μm (red) (a), 25 μm (c), and 200 μm (e).
