Fig. 3: FANCJ suppresses accumulation of G4 structures within replisomes.

a, d, g Overlaid TC triplets of EdU, G4, MCM (a), EdU, PCNA, MCM (d), or EdU, G4, MCM (g), from multiple NT or 1 h, 20 μM PDS-treated S-phase U2OS cells transfected with control (siCTRL) or FANCJ (siFANCJ) siRNA. Circle sizes of each TC triplet represents the local density of G4-Replisomes (a), EdU at All-Replisomes (d), or EdU at G4-Replisomes (g) from a given nucleus. For a, the TC triplets are aligned onto the same EdU-MCM plane to define the positions of G4 relative to the replisome complex; for d, g, the TC triples are aligned using MCM as the center to better visualize the relative magnitude of EdU. b, e, h Frequencies of G4-Replisomes (b), EdU at All-Replisomes (e), or EdU at G4-Replisomes (h) in NT or PDS-treated siCTRL or siFANCJ cells. Individual data points represent result from a single nucleus. Black horizontal line and box height indicate mean ± SD. Values on graph indicate p-values of unpaired two-sample t-tests between NT and PDS-treated cells. c, f, i Percent change in the densities of G4-Replisomes (c), EdU at All-Replisomes (f), or EdU at G4-Replisomes (i) in siCTRL or siFANCJ PDS-treated compared to NT cells. Values on the graph and black horizontal line represent the respective percent changes, box height indicates the propagated s.e.m. For all experiments, number of cells analyzed and TC triplets identified are listed in Supplementary Table 1.