Fig. 4: Deregulated G4-Replisomes resist RPA loading.

a, d Overlaid TC triplets of RPA, PCNA, MCM (a) or RPA, G4, MCM (d) from multiple NT or 4 h, 20 μM PDS-treated siCTRL or siFANCJ cells. Circle size of each TC triplet represents the local density of RPA from a given nucleus. The TC triplets are aligned onto the same PCNA-MCM (a) or G4-MCM (d) plane to define the positions of RPA relative to the replisome complex. b, e Local densities of RPA at All-Replisomes (b) or G4-Replisomes (e) in NT or PDS-treated siCTRL or siFANCJ cells. Individual data points represent result from a single nucleus. Black horizontal line and box height indicate mean ± SD. Values on graph indicate p-values of unpaired two-sample t-tests between NT and PDS-treated cells. c, f Percent change in the densities of RPA at All-Replisomes (c) or G4-Replisomes (f) in siCTRL or siFANCJ PDS-treated compared to NT cells. Values on the graph and black horizontal line represent the respective percent changes, box height indicates the propagated s.e.m. For all experiments, number of cells analyzed and TC triplets identified are listed in Supplementary Table 1.