Fig. 5: Pyrethrin repellency is the result of activation of both Or(s) and sodium channels.
From: A dual-target molecular mechanism of pyrethrum repellency against mosquitoes
a Representative single sensillum recording (SSR) traces of short sharp-tipped (sst)-1 sensilla responding to pyrethrin I (P-I; n = 8 sensilla) and pyrethrin II (P-II; n = 6 sensilla) at the 10−1 dilution from Rockefeller mosquitoes. b Repellency elicited by P-I and P-II at the 10−3 dilution in Orlando (wild-type) and orco−/− mosquitoes (n = 10 cages for Orlando control and orco−/− control from 2 batches of mosquitoes; P-I: U = 31, P = 0.034, n = 11 cages for Orlando and n = 12 cages for orco−/− from 3 batches of mosquitoes; P-II: U = 15, P < 0.001, n = 14 cages for Orlando and n = 15 cages for orco−/− from 4 batches of mosquitoes). c Repellency by pyrethrum and geranyl acetate in KDR:ROCK mosquitoes compared to that in Rockefeller mosquitoes (n = 10 cages for control in Rockefeller and n = 9 cages for control in KDR:ROCK from 2 batches of mosquitoes; pyrethrum at the 10−3 dilution: t = 2.96, df = 28, P = 0.0062, n = 15 cages for Rockefeller and n = 15 cages for KDR:ROCK from 4 batches of mosquitoes; pyrethrum at the 10−2 dilution: t = 6.56, df = 31, P < 0.0001, n = 17 cages for Rockefeller and n = 16 cages for KDR:ROCK from 4 batches of mosquitoes; geranyl acetate: t = 1.32, df = 16, P = 0.204, n = 9 cages for each strain from 2 batches of mosquitoes). Two-tailed unpaired student’s t-test or two-tailed Mann-Whitney Rank Sum test was used to compare each of two sets of data. Data are plotted as mean ± s.e.m. and dots denote the value of each repeat.
