Fig. 2: Total cellular lipid levels in absence of INPP5A. | Nature Communications

Fig. 2: Total cellular lipid levels in absence of INPP5A.

From: Inositol triphosphate-triggered calcium release blocks lipid exchange at endoplasmic reticulum-Golgi contact sites

Fig. 2

a, b, d Lipidomic analyses. SCR, control (SCR), 5AKD, INPP5A-depleted HeLa cells. Ceramide (Cer), cholesterol (Chol), cholesteryl ester (CE), diacylglycerol (DAG), dihexosylceramide (Hex2Cer), ganglioside GM1 (GM1), ganglioside GM3 (GM3), globoside Gb3 /trihexosylceramide, (Hex3Cer), hexosylceramide (HexCer), lyso-PC (LPC), phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidyinositol (PI), phosphatidylserine (PS), plasmalogen (plasmenyl) ethanolamine (pl-PE), sphingomyelin (SM), triacylglycerol (TAG). a = acyl-linked glycerophospholipids, e = ether-linked (plasmanyl) or containing one odd-chain fatty acyl. Two-sided t-test corrected for multiple comparisons using the Holm-Sidak method: aPC: p = 0.49, t = 0.69, df = 14. ePC: p = 0.98, t = 0.02, df = 14. LPC: p = 0.076, t = 1.91, df = 14. SM: p = 0.90, t = 0.1252, df = 14.aPE: p = 0.033, t = 2.35, df = 14. ePE: p = 0.005, t = 3.239, df = 14 aPS: p = 0.93, t = 0.08, df = 14. pl-PE 16:0: p = 0.077, t = 1.90, df = 14. pl-PE 18:1: p = 0.700, t = 2.29, df = 14. pl-PE 18:0: p = 0.49, t = 0.6964, df = 14. Chol: p = 0.013, t = 2.814, df = 14. CE: p = 0.644, t = 0.47, df = 14. DAG: p = 0.92, t = 0.1001, df = 14. TAG: p = 0.56, t = 0.58, df = 14. Cer: p = 0.734, t = 0.346, df = 14. HexCer: p = 0.74, t = 0.33 df = 14. Hex2Cer: p = 0.093, t = 1.79, df = 14. Hex3Cer: p = 0.007, t = 3.955, df = 6. GM3: p = 0.0002, t = 7.83, df = 6. GM1: p = 0.000034, t = 11, df = 6. c Confocal images of control (SCR) or INPP5A (5AKD)-depleted HeLa cells stained for ceramide (green). Blue, DAPI. Scale bar, 10 μm. Data are mean ± SEM from 8 (a, b), 3 (c), or 4 (d) independent experiments. For numerical source data see Source Data file.

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