Fig. 2: Highly efficient gene conversion in somatic cells revealed by using the CopyCatcher system.
a Paternal and maternal crossing schemes using either F0 males or females carrying Cas9 transgenes inserted in the yellow locus expressed by different promoters and marked with 3XP3-DsRed (in the eye). Trans-heterozygous F1 females obtained in both crosses carrying both CopyCatcher elements inserted into the white (w) or pale (ple) loci and static Cas9 expression cassettes were used to score SGC. b, d Examples of mosaic phenotypes (n = 15 biological independent flies were observed) of the F1 trans-heterozygous y+, w[ATG-,CC]/y[Cas9], w+ compound eyes (b) or y[Cas9]/+; ple[ATG-,CC]/+ thorax bristles (d). Clones of pale bristles delineated by dotted white lines in (d) denote the patches created by SGC events. c, e SGC rates measured by the fraction of F1 female progeny having double-sided mosaic eyes (c), or the fraction of pale thorax bristles relative to the total thorax bristles in individual F1 trans-heterozygous females (e). Each dot in (c) represents SGC averages from single vials (n = 10 biological independent crosses for actin promoter-driven Cas9, and n = 11 biological independent crosses for both vasa and nanos promoter-driven Cas9) and in (e) represents the percent of pale bristles for an individual fly (as shown in panel (d), n = 10 biological independent flies). ZC+ZMG: Cas9 males crossed with CopyCatcher females, ZG+ZMC: CopyCatcher males crossed with Cas9 females. Error bars indicate mean values ± SD. Asterisks represent significance with a two-tailed t-test: three asterisks (p < 0.001), two asterisks (p < 0.01), one asterisk (p < 0.05), and ns (not significant). Scale bars stand for 200 pixels. Raw data for (c) and (e) are provided as Source Data files.
