Fig. 4: Impaired proliferation of colon epithelium in Ifnar1^−/−Ifnlr1^−/− mice following DSS treatment.

a 6–8-week-old naive WT, single or double IFNR-deficient mice receiving regular water were euthanized and proliferation of colon epithelial cells were evaluated by immunohistochemistry (IHC) staining for Ki-67 (n = 5). b 6–8-week-old WT, single or double IFNR-deficient mice were exposed to 1.5% DSS in drinking water for 7 days, followed by regular drinking water for recovery. Mice were euthanized on day 8 and proliferation of colon epithelial cells was evaluated by IHC staining for Ki-67 (n = 5). Representative colon images and quantified data are shown. Scale bars are 100 μM. Data are representative of two independent experiments. Symbols represent the number of Ki-67⁺ cells per crypt (five crypt/slide/animal; n = 5). Quantitative data were analyzed using one-way ANOVA followed by Bonferroni’s multiple comparisons test and represent mean values with SEM. ****P ≤ 0.0001 and are for the indicated animal group compared with control group of WT mice.